Computer-aided measurements of cell electrorotation by image analysis techniques

Yang Yang; Chengjun Huang; Jiang Zhu; Guoliang Huang; Jing Cheng

doi:10.1117/12.741339

1 May 2007 Computer-aided measurements of cell electrorotation by image analysis techniques

Yang Yang, Chengjun Huang, Jiang Zhu, Guoliang Huang, Jing Cheng

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65342O (2007) https://doi.org/10.1117/12.741339
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

Electrorotation (ROT) is widely used for the investigation of the dielectric properties of biological cells. Traditionally the tedious measurements of the rotational rates of the cells are handled manually. In this paper, a ROT chip detection platform equipped with computer-aided measuring system is presented. The rotational motions of the cells are captured by CCD camera and an algorithm is implemented to automatically estimate the rotational rates by analyzing the captured image. The acquired data is verified by a comparison with manual stop-watch measurements. The experimental results are accurate and robust against variations in illumination and cellular deformation within maximum 5% deviation from the manual measurements. Thus the platform as a whole can be employed to detect changes in the cellular membrane dielectric properties caused by external stimulation, or those occurring naturally.

Citation Download Citation

Yang Yang, Chengjun Huang, Jiang Zhu, Guoliang Huang, and Jing Cheng "Computer-aided measurements of cell electrorotation by image analysis techniques", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65342O (1 May 2007); https://doi.org/10.1117/12.741339

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