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10 May 2007 Detection of Bacillus anthracis spores in water using biosensors based on magnetostrictive microcantilever coated with phage
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Abstract
Microcantilevers (MCs) as state-of-art sensor platforms have been widely investigated. We recently introduced a new type of MC, magnetostrictive microcantilever (MSMC), as high performance sensor platform. The MSMC is a remote/wireless sensor platform and exhibits a high quality merit factor in liquid. In this paper, a MSMC-based biosensor is developed for detecting B. anthracis spores in liquid, a potential biothreaten agent. The results demonstrated the advantages of MSMCs as a sensor platform. MSMCs with different sizes were fabricated and utilized in the experiments. The MSMCs were coated with the filamentous phage as a bio-recognition element to capture the B. anthracis spores. The phage-coated MSMCs as biosensors were exposed to cultures containing target spores with concentrations ranging from 5 * 104 spores/mL to 5 * 108 spores/mL. The resonance frequency of the MSMC sensors in cultures was monitored in a real-time manner. The results showed that for MSMCs of 2.8 mm * 1.0 mm * 35 &mgr;m and with 1.4 mm * 0.8 mm * 35 &mgr;m have a detection limit of 105 and 104 spores/mL, respectively.
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Liling Fu, Suiqiong Li, Kewei Zhang, Z.-Y. Cheng, and J.M. Barbaree "Detection of Bacillus anthracis spores in water using biosensors based on magnetostrictive microcantilever coated with phage", Proc. SPIE 6556, Micro (MEMS) and Nanotechnologies for Defense and Security, 655619 (10 May 2007); https://doi.org/10.1117/12.721004
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