Track and trap in 3D

Jesper Glückstad; Peter J. Rodrigo; Ivan P. Nielsen; Carlo A. Alonzo

doi:10.1117/12.719186

9 April 2007 Track and trap in 3D

Jesper Glückstad, Peter J. Rodrigo, Ivan P. Nielsen, Carlo A. Alonzo

Proceedings Volume 6574, Optical Pattern Recognition XVIII; 657403 (2007) https://doi.org/10.1117/12.719186
Event: Defense and Security Symposium, 2007, Orlando, Florida, United States

Abstract

Three-dimensional light structures can be created by modulating the spatial phase and polarization properties of an an expanded laser beam. A particularly promising technique is the Generalized Phase Contrast (GPC) method invented and patented at Risø National Laboratory. Based on the combination of programmable spatial light modulator devices and an advanced graphical user-interface the GPC method enables real-time, interactive and arbitrary control over the dynamics and geometry of synthesized light patterns. Recent experiments have shown that GPC-driven micro-manipulation provides a unique technology platform for fully user-guided assembly of a plurality of particles in a plane, control of particle stacking along the beam axis, manipulation of multiple hollow beads, and the organization of living cells into three-dimensional colloidal structures. Here we present GPC-based optical micromanipulation in a microfluidic system where trapping experiments are computer-automated and thereby capable of running with only limited supervision. The system is able to dynamically detect living yeast cells using a computer-interfaced CCD camera, and respond to this by instantly creating traps at positions of the spotted cells streaming at flow velocities that would be difficult for a human operator to handle.

Citation Download Citation

Jesper Glückstad, Peter J. Rodrigo, Ivan P. Nielsen, and Carlo A. Alonzo "Track and trap in 3D", Proc. SPIE 6574, Optical Pattern Recognition XVIII, 657403 (9 April 2007); https://doi.org/10.1117/12.719186

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