Fluorescence study of bovine serum albumin and Ti and Sn oxide nanoparticles interactions

Denisio M. Togashi; Alan G. Ryder; Deirdre Mc Mahon; Peter Dunne; James McManus

doi:10.1117/12.728354

11 July 2007 Fluorescence study of bovine serum albumin and Ti and Sn oxide nanoparticles interactions

Denisio M. Togashi, Alan G. Ryder, Deirdre Mc Mahon, Peter Dunne, James McManus

Proceedings Volume 6628, Diagnostic Optical Spectroscopy in Biomedicine IV; 66281K (2007) https://doi.org/10.1117/12.728354
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Nanochemistry offers stimulating opportunities for a wide variety of applications in the biosciences. Understanding of the interaction of nanoparticles with biomolecules such as proteins is very important as it can help better design and fabricate nanocomposites for applications in diagnostics, drug delivery, and cell monitoring. In this work, the interaction of Bovine Serum Albumin (BSA) and two types of metal oxide nanoparticles (titanium and tin) have been studied using the intrinsic fluorescence of tryptophan residue from the proteins measured by steady state and time resolved fluorescence techniques. The nanoparticles which were fabricated using a novel synthetic process have average sizes of ~2 nm (SnO₂) and ~6 nm (estimated for TiO₂) and have very high solubilities in a variety of solvents. The Stern-Volmer plots indicate an effective quenching process by TiO₂ nanoparticles whereas SnO₂ nanoparticles have a lower quenching efficiency for BSA fluorescence. Static quenching is the major contribution in the overall process which may indicate a high degree of association between protein and nanoparticles. The difference in BSA fluorescence quenching efficiency between the two types of nanoparticles can be explained by the non-covalent interaction differences and the thermal stability of protein-nanoparticle associated species for both materials.

Citation Download Citation

Denisio M. Togashi, Alan G. Ryder, Deirdre Mc Mahon, Peter Dunne, and James McManus "Fluorescence study of bovine serum albumin and Ti and Sn oxide nanoparticles interactions", Proc. SPIE 6628, Diagnostic Optical Spectroscopy in Biomedicine IV, 66281K (11 July 2007); https://doi.org/10.1117/12.728354

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