Development of a TIRF-FLIM microscope for biomedical applications

P. Blandin; S. Lévêque-Fort; S. Lécart; P. Zeller; Z. Lenkei; F. Druon; P. Georges

doi:10.1117/12.728429

8 August 2007 Development of a TIRF-FLIM microscope for biomedical applications

P. Blandin, S. Lévêque-Fort, S. Lécart, P. Zeller, Z. Lenkei, F. Druon, P. Georges

Proceedings Volume 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III; 66300B (2007) https://doi.org/10.1117/12.728429
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Total internal reflection fluorescence microscopy (TIRFM) is a powerful optical technique to observe fluorescence close to surfaces. Associated with fluorescence lifetime imaging, TIRFM enables to measure contrasts independent of fluorophores concentration and reveal intracellular activity with subwavelength axial resolution. We developed an original setup which allows, thanks to a wide-field time resolved detection, to measure nanoseconds fluorescence lifetimes of membrane receptors in order to apprehend their signalisation pathway.

Citation Download Citation

P. Blandin, S. Lévêque-Fort, S. Lécart, P. Zeller, Z. Lenkei, F. Druon, and P. Georges "Development of a TIRF-FLIM microscope for biomedical applications", Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300B (8 August 2007); https://doi.org/10.1117/12.728429

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