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13 July 2007 Multiphoton microscopy for the investigation of trans-cutaneous drug delivery
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The trans-cutaneous pathway for drug delivery is of particular interest since it allows a simple and non-invasive administration of pharmaceutically relevant compounds. As the skin is an effective barrier for many of these compounds, various strategies have been developed to enable and control the trans-cutaneous transport. Here we discuss, how multiphoton microscopy and spectral imaging can be valuable tools for the analysis of the penetration pathways of topically applied drugs. A time dependent study of the cutaneous penetration of a fluorescent drug model released from a nano-particular carrier is presented. The localization of single nano-particles in human skin (ex vivo) and the discrimination of different fluorescent compounds, as the drug model, the particle's label and the cutaneous endofluorescence by spectral imaging and selective excitation is shown. Multiphoton imaging techniques were found to be excellent methods for the non-invasive evaluation of cutaneous drug delivery strategies and analysis of dermal penetration pathways down to the sub-cellular level.
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Frank Stracke, Marc Schneider, Barbara Weiss, Claus-Michael Lehr, Ulrich F. Schäfer, and Karsten König "Multiphoton microscopy for the investigation of trans-cutaneous drug delivery", Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 663010 (13 July 2007);

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