Flow-assisted collection of DNA molecules on silica beads

N. Korneev; R. Ramos-García; J. C. Ramirez-San-Juan; E. Rodriguez-Aboytes; M. Cárdenas-García

doi:10.1117/12.731802

5 September 2007 Flow-assisted collection of DNA molecules on silica beads

N. Korneev, R. Ramos-García, J. C. Ramirez-San-Juan, E. Rodriguez-Aboytes, M. Cárdenas-García

Proceedings Volume 6644, Optical Trapping and Optical Micromanipulation IV; 664427 (2007) https://doi.org/10.1117/12.731802
Event: NanoScience + Engineering, 2007, San Diego, California, United States

Abstract

We demonstrate the possibility to collect DNA molecules from solution on beads trapped by a focused laser beam close to the cell bottom. The combined action of laser-induced flow and laser pressure keeps the bead in place while the flow deposits the DNA. We show that the combined action of walls and volume absorption can control the direction and magnitude of the induced convention flow. We report trapping of viral bacterial DNA on micrometer size beads with laser power in the mW range.

Citation Download Citation

N. Korneev, R. Ramos-García, J. C. Ramirez-San-Juan, E. Rodriguez-Aboytes, and M. Cárdenas-García "Flow-assisted collection of DNA molecules on silica beads", Proc. SPIE 6644, Optical Trapping and Optical Micromanipulation IV, 664427 (5 September 2007); https://doi.org/10.1117/12.731802

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