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The collecting and sorting micro size particles by electric force is easy to integrate with other bioassays. There are many
forms of electric forces such as electrophoresis, dielectrophoresis and electroosmosis which can be used to manipulate
particles. In an attempt to understand the role of electroosmosis and dielectrophoresis in the collection of micro size
particles, a small device made of two parallel plates is used to study the particle movement under AC electric field. The
device is fabricated by a top electrode and a bottom electrode separated by a spacer. The top electrode is made from an
ITO glass where the bottom electrode is made of Corning 1737 glass sputtered with chromium. A dielectric layer is
fabricated by spin coating a thin photo-resist (0.5~1μm) on the bottom electrode and a spacer made of curing PDMS is
utilized to separate these electrodes. A 900μm × 900μm collecting chamber is fabricated on the bottom electrode via
photolithography. The amine-modified polystyrene fluorescent particles whose average size is 1 μm were used for
collection experiments. Different frequency and power were applied to generate the non-uniform electric field. It was
found that frequency is the critical factor for electroosmotic velocity. There seems to be an optimum frequency that leads
to largest particle velocity. The underlying mechanism is believed to the competing forces among dielectrophoresis and
electroosmosis. This device demonstrates that the electroosmosis force is suitable for collecting bio-particles in AC
electric field.
Chehung Wei,Che-Wei Hsu, andChing-Chieh Wang
"The role of electro-osmosis and dielectrophoresis in collection of micro/nano size particles in low frequency AC electric field", Proc. SPIE 6645, Nanoengineering: Fabrication, Properties, Optics, and Devices IV, 664528 (11 September 2007); https://doi.org/10.1117/12.735185
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Chehung Wei, Che-Wei Hsu, Ching-Chieh Wang, "The role of electro-osmosis and dielectrophoresis in collection of micro/nano size particles in low frequency AC electric field," Proc. SPIE 6645, Nanoengineering: Fabrication, Properties, Optics, and Devices IV, 664528 (11 September 2007); https://doi.org/10.1117/12.735185