Quantum dot-based western blot for sensitive detection of pig serum antibody to <i>actinobacillus pleuropneumoniae</i>

Ana Cişmileanu; Cornelia Sima; Constantin Grigoriu

doi:10.1117/12.756829

1 August 2007 Quantum dot-based western blot for sensitive detection of pig serum antibody to actinobacillus pleuropneumoniae

Ana Cişmileanu, Cornelia Sima, Constantin Grigoriu

Author Affiliations +

Proceedings Volume 6785, ROMOPTO 2006: Eighth Conference on Optics; 67850J (2007) https://doi.org/10.1117/12.756829
Event: ROMOPTO 2006: Eighth Conference on Optics, 2006, Sibiu, Romania

Abstract

A quantum dot - immunoglobulin conjugate specific for pig IgG, was obtained by carbodiimide chemistry. We used a Western blot technique for detecting specific antibodies against Actinobacillus pleuropneumoniae (A. pp), which cause porcine pleuropneumonia. The antigen used in this technique was Apx haemolysin which is an important virulence factor of A. pp and it induces protective immunity in vaccined pigs. The detection on Western blot membrane was possible at 1/50 dilution of quantum dot conjugate at a dilution of pig serum till 1/6400. The results for pig serum demonstrated a higher sensitivity of QD-based Western blot technique for the presence of antibodies specific for Apx haemolysin in comparison with similar classical techniques (with coloured substrate for enzyme present in secondary antibody conjugate).

Citation Download Citation

Ana Cişmileanu, Cornelia Sima, and Constantin Grigoriu "Quantum dot-based western blot for sensitive detection of pig serum antibody to actinobacillus pleuropneumoniae", Proc. SPIE 6785, ROMOPTO 2006: Eighth Conference on Optics, 67850J (1 August 2007); https://doi.org/10.1117/12.756829

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