Identification of endogenous fluorophores in the photoreceptors using autofluorescence spectroscopy

Lingling Zhao; Junle Qu D.D.S.; Hanben Niu

doi:10.1117/12.760139

8 January 2008 Identification of endogenous fluorophores in the photoreceptors using autofluorescence spectroscopy

Lingling Zhao, Junle Qu D.D.S., Hanben Niu

Proceedings Volume 6826, Optics in Health Care and Biomedical Optics III; 682614 (2008) https://doi.org/10.1117/12.760139
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

In this paper, we present our investigation on the identification of endogenous fluorophores in photoreceptors using autofluorescence spectroscopy, which is performed with an inverted laser scanning confocal microscope equipped with an Argon ion laser and a GreNe laser. In our experiments, individual cones and rods are clearly resolved even in freshly prepared retina samples, without slicing or labeling. The experiment results show that autofluorescence spectrum of the photoreceptors has three peaks approximately at 525nm, 585nm and 665nm. Furthermore, the brightest autofluorescence originates from the photoreceptor outer segments. We can, therefore, come to a conclusion that the peaks at 525nm, 585nm are corresponding to FAD and A2-PE, respectively, which are distributed in the photoreceptor outer segments.

Citation Download Citation

Lingling Zhao, Junle Qu D.D.S., and Hanben Niu "Identification of endogenous fluorophores in the photoreceptors using autofluorescence spectroscopy", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 682614 (8 January 2008); https://doi.org/10.1117/12.760139

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