Non-invasive analysis/diagnosis of human normal and melanoma skin tissues with two-photon FLIM in vivo

Iris Riemann; Alexander Ehlers; Ronan Le Harzic; Enrico Dimitrow; Martin Kaatz; P. Elsner; Rainer Bückle; Karsten König

doi:10.1117/12.762937

8 February 2008 Non-invasive analysis/diagnosis of human normal and melanoma skin tissues with two-photon FLIM in vivo

Iris Riemann, Alexander Ehlers, Ronan Le Harzic, Enrico Dimitrow, Martin Kaatz, P. Elsner, Rainer Bückle, Karsten König

Author Affiliations +

Proceedings Volume 6842, Photonic Therapeutics and Diagnostics IV; 684205 (2008) https://doi.org/10.1117/12.762937
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Multiphoton optical tomography provides non-invasive optical sectioning of skin with high spatial and intracellular resolution. The imaging system DermaInspect based on femtosecond laser pulses and equipped with a SPC 830 card for FLIM analysis was used to perform this technique in vivo in clinical studies on patients with abnormal pigmented tissues. After examination in vivo, biopsies were taken and analyzed again. So far it was possible to distinguish between normal and diseased tissues mainly due to their different morphological appearances in the imaging mode: melanoma tissues show differences in the structure of the epidermal layers and the presence of dendritic cells compared to normal skin. The optical analysis can be improved when using the system in the time-correlated single photon counting mode. Fluorescence lifetime analysis reveals characteristic differences between healthy and melanoma tissues even before the visible morphological changes in human tissue in vivo.

Citation Download Citation

Iris Riemann, Alexander Ehlers, Ronan Le Harzic, Enrico Dimitrow, Martin Kaatz, P. Elsner, Rainer Bückle, and Karsten König "Non-invasive analysis/diagnosis of human normal and melanoma skin tissues with two-photon FLIM in vivo", Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 684205 (8 February 2008); https://doi.org/10.1117/12.762937

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available