Scatter sensitive microscopic techniques to identify contrasting mucosal structures in ultrahigh-resolution optical coherence tomograms of mouse colon

Alexandre R. Tumlinson; Lida P. Hariri; Wolfgang Drexler; Jennifer K. Barton

doi:10.1117/12.763968

21 February 2008 Scatter sensitive microscopic techniques to identify contrasting mucosal structures in ultrahigh-resolution optical coherence tomograms of mouse colon

Alexandre R. Tumlinson, Lida P. Hariri, Wolfgang Drexler, Jennifer K. Barton

Author Affiliations +

Proceedings Volume 6851, Endoscopic Microscopy III; 68510A (2008) https://doi.org/10.1117/12.763968
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Optical coherence tomography, optical coherence microscopy, reflectance confocal microscopy, and darkfield microscopy all derive contrast from the intensity of endogenous tissue scatter. We have imaged excised mouse colon tissue with these complimentary technologies to make conclusions about structural origins of scatter in the mouse colonic mucosa observed with endoscopic OCT. We find hyperintense scattering both from the cytoplasm of epithelial cells and from the boundary between epithelia and the lamina propria. We find almost no scatter from the portion of epithelial cells containing the nucleus. These observations substantiate explanations for the appearance of colonic crypts and the luminal surface.

Citation Download Citation

Alexandre R. Tumlinson, Lida P. Hariri, Wolfgang Drexler, and Jennifer K. Barton "Scatter sensitive microscopic techniques to identify contrasting mucosal structures in ultrahigh-resolution optical coherence tomograms of mouse colon", Proc. SPIE 6851, Endoscopic Microscopy III, 68510A (21 February 2008); https://doi.org/10.1117/12.763968

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