Mucosal layer as major source of green autofluorescence in the colon under excitation by blue light

Keimei Nakano; Yoshinori Harada; Yoshihisa Yamaoka; Kiichiro Miyawaki; Naoki Wakabayashi; Shoji Mitsufuji; Katsuichi Imaizumi; Hideyuki Takaoka; Masaya Nakaoka; Tetsuro Takamatsu

doi:10.1117/12.763245

8 February 2008 Mucosal layer as major source of green autofluorescence in the colon under excitation by blue light

Keimei Nakano, Yoshinori Harada, Yoshihisa Yamaoka, Kiichiro Miyawaki, Naoki Wakabayashi, Shoji Mitsufuji, Katsuichi Imaizumi, Hideyuki Takaoka, Masaya Nakaoka, Tetsuro Takamatsu

Author Affiliations +

Proceedings Volume 6853, Biomedical Optical Spectroscopy; 68531H (2008) https://doi.org/10.1117/12.763245
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Autofluorescence endoscopy is a promising modality for diagnosis of colonic tumors. This article discusses the origin of autofluorescence of the normal colon. Excised normal colons were analyzed by using fluorescence stereomicroscopy and a fluorescence-lifetime microscopy system. Fluorescence images showed that the mucosa had stronger autofluorescence than the submucosa. The results of fluorescence-lifetime measurement showed that nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) might be responsible for the autofluorescence of the colonic epithelia. Our results suggest that the mucosal autofluorescence generates by NADH and FAD was an important source of the green autofluorescence.

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Keimei Nakano, Yoshinori Harada, Yoshihisa Yamaoka, Kiichiro Miyawaki, Naoki Wakabayashi, Shoji Mitsufuji, Katsuichi Imaizumi, Hideyuki Takaoka, Masaya Nakaoka, and Tetsuro Takamatsu "Mucosal layer as major source of green autofluorescence in the colon under excitation by blue light", Proc. SPIE 6853, Biomedical Optical Spectroscopy, 68531H (8 February 2008); https://doi.org/10.1117/12.763245

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