Viability evaluation of culture cells patterned by femtosecond laser-induced impulsive force

Noriko Takizawa; Kazunori Okano; Takayuki Uwada; Yoichiroh Hosokawa; Hiroshi Masuhara

doi:10.1117/12.762584

20 February 2008 Viability evaluation of culture cells patterned by femtosecond laser-induced impulsive force

Noriko Takizawa, Kazunori Okano, Takayuki Uwada, Yoichiroh Hosokawa, Hiroshi Masuhara

Proceedings Volume 6854, Optical Interactions with Tissue and Cells XIX; 685411 (2008) https://doi.org/10.1117/12.762584
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

PC12 cells, which are derived from a rat pheochromocytoma, were independently patterned utilizing an impulsive force resulting in impulsive shockwave and cavitation bubble generation by focused femtosecond laser irradiation. Since the PC12 cells respond reversibly to nerve growth factor by induction of the neuronal phenotype, we can assess an influence that the impulsive force gives to the bioactivity in term of the cell differentiation. The patterned cells were accumulated on an intact dish and cultured for 3 days. The behavior of appearance and cell differentiation was observed by multipoint time-lapse system. On bases of these results, it was proved that the biological activity of the cell is unaffected by the femtosecond laser patterning.

Citation Download Citation

Noriko Takizawa, Kazunori Okano, Takayuki Uwada, Yoichiroh Hosokawa, and Hiroshi Masuhara "Viability evaluation of culture cells patterned by femtosecond laser-induced impulsive force", Proc. SPIE 6854, Optical Interactions with Tissue and Cells XIX, 685411 (20 February 2008); https://doi.org/10.1117/12.762584

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