Simultaneous imaging of two initiator caspases during cisplatin-induced HeLa apoptosis

Jun Chu; Liang Wang; Qingming Luo; Zhihong Zhang D.V.M.

doi:10.1117/12.767864

18 February 2008 Simultaneous imaging of two initiator caspases during cisplatin-induced HeLa apoptosis

Jun Chu, Liang Wang, Qingming Luo, Zhihong Zhang D.V.M.

Proceedings Volume 6857, Biophotonics and Immune Responses III; 68570R (2008) https://doi.org/10.1117/12.767864
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Caspase-2 is an initiating caspase required for stress-induced apoptosis in various human cancer cells. Activation of caspase-9, a key event in stress-mediated apoptosis, also has been shown to be an initiator caspase. However, the timing or activation sequence of these initiator caspases, which trigger apoptotic pathway, is unclear. Here we report caspase-2 and caspase-9 dynamics during cisplatin-induced HeLa apoptosis using Double Fluorescence Resonance Energy Transfer (FRET) technique. Two FRET probes were constructed that each encoded a CRS (caspase-2 or caspase-9 recognition Site) fused with a cyan/yellow fluorescent protein (CFP/YFP) and a red fluorescent protein (DsRed) (CFP/YFP-CRS-DsRed). By using two probes, CFP-C2-DsRed and YFP-C9-DsRed, we carried out simultaneous double-FRET analysis and revealed that activation of caspase-2 had the same time course with caspase-9. These data suggest parallel activation of initiator caspase-2 and caspase-9 in cisplatin-induced cell death.

Citation Download Citation

Jun Chu, Liang Wang, Qingming Luo, and Zhihong Zhang D.V.M. "Simultaneous imaging of two initiator caspases during cisplatin-induced HeLa apoptosis", Proc. SPIE 6857, Biophotonics and Immune Responses III, 68570R (18 February 2008); https://doi.org/10.1117/12.767864

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