Selective labeling of a single organelle by using two-photon conversion of a photoconvertible fluorescent protein

Wataru Watanabe; Tomoko Shimada; Sachihiro Matsunaga; Daisuke Kurihara; Shin-ichi Arimura; Nobuhiro Tsutsumi; Kiichi Fukui; Kazuyoshi Itoh

doi:10.1117/12.768745

15 February 2008 Selective labeling of a single organelle by using two-photon conversion of a photoconvertible fluorescent protein

Wataru Watanabe, Tomoko Shimada, Sachihiro Matsunaga, Daisuke Kurihara, Shin-ichi Arimura, Nobuhiro Tsutsumi, Kiichi Fukui, Kazuyoshi Itoh

Author Affiliations +

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 68601B (2008) https://doi.org/10.1117/12.768745
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We present space-selective labeling of organelles by using two-photon conversion of a photoconvertible fluorescent protein with near-infrared femtosecond laser pulses. Two-photon excitation of photoconvertible fluorescent-protein, Kaede, enables space-selective labeling of organelles. We alter the fluorescence of target mitochondria in a tobacco BY-2 cell from green to red by focusing femtosecond laser pulses with a wavelength of 750 nm.

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Wataru Watanabe, Tomoko Shimada, Sachihiro Matsunaga, Daisuke Kurihara, Shin-ichi Arimura, Nobuhiro Tsutsumi, Kiichi Fukui, and Kazuyoshi Itoh "Selective labeling of a single organelle by using two-photon conversion of a photoconvertible fluorescent protein", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601B (15 February 2008); https://doi.org/10.1117/12.768745

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