Serotonin: multiphoton imaging and relevant spectral data

S. K. Kaushalya; Suman Nag; J. Balaji; S. Maiti

doi:10.1117/12.763020

15 February 2008 Serotonin: multiphoton imaging and relevant spectral data

S. K. Kaushalya, Suman Nag, J. Balaji, S. Maiti

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 68601C (2008) https://doi.org/10.1117/12.763020
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Coupling three-photon microscopy with automated stage movement can now produce a live high resolution map of the neurotransmitter serotonin in a single cross section of the whole rat brain. Accurate quantification of these serotonin images demands appropriate spectral filtering. This requires one to consider that the spectral characteristics of serotonin show a remarkable variation as it non-covalently associates with different molecules, as we discuss here. Also it is known that serotonin emission changes when it forms a covalent adduct with para-formaldehyde. This provides a potential route for producing a whole brain serotonin map using multiphoton microscopy in a fixed rat brain. Here we take the initial step showing that multiphoton microscopy of this adduct can quantitatively image chemically induced changes in serotonin distribution.

Citation Download Citation

S. K. Kaushalya, Suman Nag, J. Balaji, and S. Maiti "Serotonin: multiphoton imaging and relevant spectral data", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601C (15 February 2008); https://doi.org/10.1117/12.763020

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available