A video rate laser scanning confocal microscope

Hongzhou Ma; James Jiang; Hongwu Ren; Alex E. Cable

doi:10.1117/12.761709

12 February 2008 A video rate laser scanning confocal microscope

Hongzhou Ma, James Jiang, Hongwu Ren, Alex E. Cable

Proceedings Volume 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV; 68610D (2008) https://doi.org/10.1117/12.761709
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

A video-rate laser scanning microscope was developed as an imaging engine to integrate with other photonic building blocks to fulfill various microscopic imaging applications. The system is quipped with diode laser source, resonant scanner, galvo scanner, control electronic and computer loaded with data acquisition boards and imaging software. Based on an open frame design, the system can be combined with varies optics to perform the functions of fluorescence confocal microscopy, multi-photon microscopy and backscattering confocal microscopy. Mounted to the camera port, it allows a traditional microscope to obtain confocal images at video rate. In this paper, we will describe the design principle and demonstrate examples of applications.

Citation Download Citation

Hongzhou Ma, James Jiang, Hongwu Ren, and Alex E. Cable "A video rate laser scanning confocal microscope", Proc. SPIE 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV, 68610D (12 February 2008); https://doi.org/10.1117/12.761709

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KEYWORDS

Confocal microscopy

Scanners

Video

Laser scanners

Luminescence

Imaging systems

Objectives

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