Controlled assembly of gold nanoparticles using De Novo designed polypeptide scaffolds

Daniel Aili; Hsu Shu Han; Karin Enander; Lars Baltzer; Bo Liedberg

doi:10.1117/12.775806

20 February 2008 Controlled assembly of gold nanoparticles using De Novo designed polypeptide scaffolds

Daniel Aili, Hsu Shu Han, Karin Enander, Lars Baltzer, Bo Liedberg

Proceedings Volume 6885, MEMS/MOEMS Components and Their Applications V. Special Focus Topics: Transducers at the Micro-Nano Interface; 688506 (2008) https://doi.org/10.1117/12.775806
Event: MOEMS-MEMS 2008 Micro and Nanofabrication, 2008, San Jose, California, United States

Abstract

Heterodimerization between designed helix-loop-helix polypeptides was utilized in order to assemble gold nanoparticles on planar substrates. The peptides were designed to fold into four-helix bundles upon dimerization. A Cys-residue in the loop region was used to immobilize one of the complementary peptides on a maleimide containing SAM on planar gold substrates whereas the second peptide was immobilized directly on gold nanoparticles. Introducing the peptide decorated particles over a peptide functionalized surface resulted in particle assembly. Further, citrate stabilized particles were assembled on amino-silane modified glass and silicon substrates. By subsequently introducing peptides and gold nanoparticles, particle-peptide hybrid multi layers could be formed.

Citation Download Citation

Daniel Aili, Hsu Shu Han, Karin Enander, Lars Baltzer, and Bo Liedberg "Controlled assembly of gold nanoparticles using De Novo designed polypeptide scaffolds", Proc. SPIE 6885, MEMS/MOEMS Components and Their Applications V. Special Focus Topics: Transducers at the Micro-Nano Interface, 688506 (20 February 2008); https://doi.org/10.1117/12.775806

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