Glycoprotein expression by adenomatous polyps of the colon

Celeste A. Roney; Jianwu Xie; Biying Xu; Paul Jabour; Gary Griffiths; Ronald M. Summers M.D.

doi:10.1117/12.769224

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12 March 2008 Glycoprotein expression by adenomatous polyps of the colon

Celeste A. Roney, Jianwu Xie, Biying Xu, Paul Jabour, Gary Griffiths, Ronald M. Summers M.D.

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Proceedings Volume 6916, Medical Imaging 2008: Physiology, Function, and Structure from Medical Images; 69161O (2008) https://doi.org/10.1117/12.769224
Event: Medical Imaging, 2008, San Diego, California, United States

Abstract

Colon cancer is the second leading cause of cancer related deaths in the United States. Specificity in diagnostic imaging for detecting colorectal adenomas, which have a propensity towards malignancy, is desired. Adenomatous polyp specimens of the colon were obtained from the mouse model of colorectal cancer called adenomatous polyposis coli-multiple intestinal neoplasia (APC^Min). Histological evaluation, by the legume protein Ulex europaeus agglutinin I (UEA-1), determined expression of the glycoprotein α-L-fucose. FITC-labelled UEA-1 confirmed overexpression of the glycoprotein by the polyps on fluorescence microscopy in 17/17 cases, of which 13/17 included paraffin-fixed mouse polyp specimens. In addition, FITC-UEA-1 ex vivo multispectral optical imaging of 4/17 colonic specimens displayed over-expression of the glycoprotein by the polyps, as compared to non-neoplastic mucosa. Here, we report the surface expression of α-L-fucosyl terminal residues by neoplastic mucosal cells of APC specimens of the mouse. Glycoprotein expression was validated by the carbohydrate binding protein UEA-1. Future applications of this method are the development of agents used to diagnose cancers by biomedical imaging modalities, including computed tomographic colonography (CTC). UEA-1 targeting to colonic adenomas may provide a new avenue for the diagnosis of colorectal carcinoma by CT imaging.

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Celeste A. Roney, Jianwu Xie, Biying Xu, Paul Jabour, Gary Griffiths, and Ronald M. Summers M.D. "Glycoprotein expression by adenomatous polyps of the colon", Proc. SPIE 6916, Medical Imaging 2008: Physiology, Function, and Structure from Medical Images, 69161O (12 March 2008); https://doi.org/10.1117/12.769224

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