A multitechnique study of bacteriorhodopsin's photonics toward new optical devices

Marta Martin; Marie-Belle Saab; Thierry Cloitre; Elias Estephan; René Legros; Frédéric J. G. Cuisinier; László Zimányi; Csilla Gergely

doi:10.1117/12.781016

8 May 2008 A multitechnique study of bacteriorhodopsin's photonics toward new optical devices

Marta Martin, Marie-Belle Saab, Thierry Cloitre, Elias Estephan, René Legros, Frédéric J. G. Cuisinier, László Zimányi, Csilla Gergely

Author Affiliations +

Proceedings Volume 6991, Biophotonics: Photonic Solutions for Better Health Care; 69910A (2008) https://doi.org/10.1117/12.781016
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

Bacteriorhodopsin (BR) is a robust trans-membrane protein that functions as a light-driven proton pump, thus is an excellent candidate for biophotonics applications. For the development of new optical devices, the buildup of stable BR matrices has to be optimised. In this work, we present a multi- technique approach: the combination of optical waveguide lightmode spectroscopy (OWLS), atomic force microscopy (AFM) and multi-photon microscopy (MPM) aiming to analyze the optical and physico-chemical properties of BR embedded in polyelectrolyte multilayers (PEM) in its membrane bound form (purple membrane, PM), as well as solubilized BR immobilized within a photonic structure built of porous silicon (PSi). OWLS measurements revealed the possibility of incorporation of PM-BR layers into PE-multilayers. The calculated thickness and refractive index of the adsorbed layers demonstrate the successful adsorption of PM on top of the positively or negatively charged PE layers. Morphological studies by AFM proved a complete coverage of the positively charged PE layer with PM patches. As for the other model system, photonic responses of BR, after being immobilized within PSi substrates, have been evaluated using multi-photon microscopy. Fluorescence emission and second harmonic generation (SHG) of the BR-PSi system were observed at some particular pores of PSi and subsequent enhancement of the signal arising from the BR adsorbed within the pores was detected. Our results constitute the first steps of two interesting and innovative biomimetic approaches for the future design and development of BR based integrated optical devices.

Citation Download Citation

Marta Martin, Marie-Belle Saab, Thierry Cloitre, Elias Estephan, René Legros, Frédéric J. G. Cuisinier, László Zimányi, and Csilla Gergely "A multitechnique study of bacteriorhodopsin's photonics toward new optical devices", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69910A (8 May 2008); https://doi.org/10.1117/12.781016

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available