Dynamically monitoring the gene expression of dual fluorophore in the cell cycle with quantitative spectrum analysis

Ja-Yun Lee; Tzong-Yuan Wu; I-Jen Hsu

doi:10.1117/12.781602

2 May 2008 Dynamically monitoring the gene expression of dual fluorophore in the cell cycle with quantitative spectrum analysis

Ja-Yun Lee, Tzong-Yuan Wu, I-Jen Hsu

Author Affiliations +

Proceedings Volume 6991, Biophotonics: Photonic Solutions for Better Health Care; 699113 (2008) https://doi.org/10.1117/12.781602
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

The cloning and transcription techniques on gene cloned fluorescent proteins have been widely used in many applications. They have been used as reporters of some conditions in a series of reactions. However, it is usually difficult to monitor the specific target with the exactly number of proteins during the process in turbid media, especially at micrometer scales. We successfully revealed an alternative way to monitor the cell cycle behavior and quantitatively analyzed the target cells with green and red fluorescent proteins (GFP and RFP) during different phases of the cell cycle by quantitatively analyzing its behavior and also monitoring its spatial distribution.

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Ja-Yun Lee, Tzong-Yuan Wu, and I-Jen Hsu "Dynamically monitoring the gene expression of dual fluorophore in the cell cycle with quantitative spectrum analysis", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 699113 (2 May 2008); https://doi.org/10.1117/12.781602

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