Design of specific biochips for contrast enhancement of UV biological absorption

Kristelle Robin; Jean-Luc Reverchon; Laurent Mugherli; Henri Benisty

doi:10.1117/12.781452

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9 May 2008 Design of specific biochips for contrast enhancement of UV biological absorption

Kristelle Robin, Jean-Luc Reverchon, Laurent Mugherli, Henri Benisty

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Proceedings Volume 6991, Biophotonics: Photonic Solutions for Better Health Care; 69912A (2008) https://doi.org/10.1117/12.781452
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

This paper describes the design of biochips enhancing absorption in UV (and more particularly at 260-280 nm). These contrast-enhancing multilayers structures are developed in order to image biological compounds immobilized at a surface. The measurement set-up is a reflection set-up, including a previously developed AlGaN detector, spectrally selective at the wavelength of biological compounds absorption (260 nm for DNA and 280 nm for proteins). A contrast study is carried out using an inorganic absorber (titanium dioxide), enabling an optimized electromagnetic interaction with the absorber. The biochip design is based on an aluminum mirror covered with a transparent dielectric. To account for roughness and oxidation, the material is modeled by a thin layer of effective medium defined in the Bruggeman approximation. We discuss the absorption expected from various biological compounds, and the capability of our set-up for detection of monolayers of DNA or protein molecules.

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Kristelle Robin, Jean-Luc Reverchon, Laurent Mugherli, and Henri Benisty "Design of specific biochips for contrast enhancement of UV biological absorption", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69912A (9 May 2008); https://doi.org/10.1117/12.781452

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