Fluorescent analysis in cell monitoring of environment and human health

Natalia A. Karnaukhova; Larisa A. Sergievich; Valery N. Karnaukhov

doi:10.1117/12.802309

29 April 2008 Fluorescent analysis in cell monitoring of environment and human health

Natalia A. Karnaukhova, Larisa A. Sergievich, Valery N. Karnaukhov

Proceedings Volume 7006, Lasers for Measurements and Information Transfer 2007; 700613 (2008) https://doi.org/10.1117/12.802309
Event: Lasers for Measurements and Information Transfer 2007, 2007, St. Petersburg, Russian Federation

Abstract

One of the important methods to study the intracellular regulation of metabolism is the fluorescent spectroscopy. Fluorochrome acridine orange (AO) is widely used to investigate the nucleic acids in living as well as fixed cells. The objective of this research was to study the functional activity of the cell synthesizing apparatus with spectral fluorescent method. The cell functional (synthetic) activity is described by the parameter α representing the RNA/DNA ratio in fixed cells stained with AO. The possibilities of fluorescent cytodiagnosis have been studied to analyze the blood system cells in some diseases, under ionizing and electromagnetic irradiations, in natural and artificial hypomethabolic states as well as nervous and reproductive systems in the mollusks.

Citation Download Citation

Natalia A. Karnaukhova, Larisa A. Sergievich, and Valery N. Karnaukhov "Fluorescent analysis in cell monitoring of environment and human health", Proc. SPIE 7006, Lasers for Measurements and Information Transfer 2007, 700613 (29 April 2008); https://doi.org/10.1117/12.802309

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