Patterning and characterization of model phospholipid membranes

Aschalew Kassu; Fernando A. Calzzani Jr.; Jean M. Taguenang; Redahegn K. Sileshi; Anup Sharma

doi:10.1117/12.794377

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28 August 2008 Patterning and characterization of model phospholipid membranes

Aschalew Kassu, Fernando A. Calzzani Jr., Jean M. Taguenang, Redahegn K. Sileshi, Anup Sharma

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Proceedings Volume 7035, Biosensing; 703508 (2008) https://doi.org/10.1117/12.794377
Event: NanoScience + Engineering, 2008, San Diego, California, United States

Abstract

Phospholipid, which is a building block of biological membranes, plays an important role in compartmentalization of cellular reaction environment and control of the physicochemical conditions inside the reaction environment. Phospholipid bilayer membrane has been proposed as a natural biocompatible platform for attaching biological molecules like proteins for biosensing related application. Due to the enormous potential applications of biomimetic model biomembranes, various techniques for depositions and patterning of these membranes onto solid supports and their possible biotechnological applications have been reported by different groups. In this work, patterning of phospholipid thin-films is accomplished by interferometric lithography as well as using lithographic masks in liquid phase. Surface Enhanced Raman Spectroscopy and Atomic Force microscopy are used to characterize the model phospholipid membrane and the patterning technique. We describe an easy and reproducible technique for direct patterning of azo-dye (NBD)-labeled phospholipid (phosphatidylcholine) in aqueous medium using a low-intensity 488 nm Ar⁺ laser and various kinds of lithographic masks.

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Aschalew Kassu, Fernando A. Calzzani Jr., Jean M. Taguenang, Redahegn K. Sileshi, and Anup Sharma "Patterning and characterization of model phospholipid membranes", Proc. SPIE 7035, Biosensing, 703508 (28 August 2008); https://doi.org/10.1117/12.794377

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