Direct concentration of circulating DNA by using a nanostructured tip

Woon-Hong Yeo; Jae-Hyun Chung; Yaling Liu; Kyong-Hoon Lee

doi:10.1117/12.795098

29 August 2008 Direct concentration of circulating DNA by using a nanostructured tip

Woon-Hong Yeo, Jae-Hyun Chung, Yaling Liu, Kyong-Hoon Lee

Proceedings Volume 7035, Biosensing; 70350N (2008) https://doi.org/10.1117/12.795098
Event: NanoScience + Engineering, 2008, San Diego, California, United States

Abstract

Nucleic acids circulating in body fluids are drawing great attention due to their potential use for disease diagnostics and prognostics. Current detection methods have yet to demonstrate the capability to selectively detect the low abundance nucleic acids. The challenge lies in the separation of circulating DNA from the genomic DNA in normal cells. Here we present an approach employing a nano-structured tip, which directly concentrates nucleic acids to the tip from a sample solution. The high aspect ratio tip is able to collect nucleic acid molecules out of a buffer solution by using dielectrophoretic (DEP) and surface tension force. The DEP force attracts DNA and other biomolecules in the vicinity of a nanotip from the sample solution. Among the attracted molecules, circulating nucleic acids whose dimensions are much smaller than the nanotip diameter are selectively captured to the tip while other bioparticles comparable to or larger than the tip diameter remain in the solution due to surface tension induced force. The concentrated DNA molecules are characterized by SEM, X-ray, and fluorescent microscopy, which demonstrate DNA capturing out of a sample solution having a 1pg/mL DNA concentration. The nanotip-based capturing method will facilitate rapid, but highly sensitive detection of circulating DNA directly from minimally treated- or raw samples.

Citation Download Citation

Woon-Hong Yeo, Jae-Hyun Chung, Yaling Liu, and Kyong-Hoon Lee "Direct concentration of circulating DNA by using a nanostructured tip", Proc. SPIE 7035, Biosensing, 70350N (29 August 2008); https://doi.org/10.1117/12.795098

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available