Three-dimensional spatial localization of thin fluorophore-filled capillaries in thick scattering media

Johanne Desrochers; Patrick Vermette; Réjean Fontaine; Yves Bérubé-Lauzière

doi:10.1117/12.807556

12 August 2008 Three-dimensional spatial localization of thin fluorophore-filled capillaries in thick scattering media

Johanne Desrochers, Patrick Vermette, Réjean Fontaine, Yves Bérubé-Lauzière

Proceedings Volume 7099, Photonics North 2008; 709908 (2008) https://doi.org/10.1117/12.807556
Event: Photonics North 2008, 2008, Montréal, Canada

Abstract

Fluorescence optical diffuse tomography (fDOT) is of much interest in molecular imaging to retrieve information from fluorescence signals emitted from specifically targeted bioprocesses deep within living tissues. An exciting application of fDOT is in the growing field of tissue engineering, where 3D non-invasive imaging techniques are required to ultimately grow 3D engineered tissues. Via appropriate labelling strategies and fluorescent probes, fDOT has the potential to monitor culture environment and cells viability non-destructively directly within the bioreactor environment where tissues are to be grown. Our ultimate objective is to image the formation of blood vessels in bioreactor conditions. Herein, we use a non-contact setup for small animal fDOT imaging designed for 3D light collection around the sample. We previously presented a time of flight approach using a numerical constant fraction discrimination technique to assign an early photons arrival time to every fluorescence time point-spread function collected around the sample. Towards bioreactor in-situ imaging, we have shown the capability of our approach to localize a fluorophore-filled 500 μm capillary immersed coaxially in a cylindrically shaped bioreactor phantom containing an absorbing/scattering medium representative of experiments on real tissue cultures. Here, we go one step further, and present results for the 3D localization of thinner indocyanine green labelled capillaries (250 μm and 360 μm inner diameter) immersed in the same phantom conditions and geometry but with different spatial configurations (10° and 30° capillary inclination).

Citation Download Citation

Johanne Desrochers, Patrick Vermette, Réjean Fontaine, and Yves Bérubé-Lauzière "Three-dimensional spatial localization of thin fluorophore-filled capillaries in thick scattering media", Proc. SPIE 7099, Photonics North 2008, 709908 (12 August 2008); https://doi.org/10.1117/12.807556

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