Two-species microparticle detection in optofluidic biochips with polymeric waveguides

H. Hosseinkhannazer; L. W. Kostiuk; J. N. McMullin

doi:10.1117/12.807238

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12 August 2008 Two-species microparticle detection in optofluidic biochips with polymeric waveguides

H. Hosseinkhannazer, L. W. Kostiuk, J. N. McMullin

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Proceedings Volume 7099, Photonics North 2008; 70990H (2008) https://doi.org/10.1117/12.807238
Event: Photonics North 2008, 2008, Montréal, Canada

Abstract

The fabrication and experimental testing of polydimethylsiloxane (PDMS) optofluidic biochips with integrated solidcore polyepoxyacrylate (PEA). optical waveguides is described. The biochips were replicated from silicon masters made by 50-μm-deep reactive ion etching. Each biochip contained three 70-μm-wide solid-core waveguides focused on the middle section of a 50-μm-wide, Y-shaped, microchannel. Electroosmotic flow with a voltage range of 50 to 300 volts was used to drive fluorescent beads in the microfluidic channel. Using two excitation lasers at 532nm and 635nm with distinct modulation frequencies, two species of microparticles with different fluorophores were identified by capturing the fluorescence in a photomultiplier tube and analyzing the signal with a windowed Fourier transform analysis technique.

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H. Hosseinkhannazer, L. W. Kostiuk, and J. N. McMullin "Two-species microparticle detection in optofluidic biochips with polymeric waveguides", Proc. SPIE 7099, Photonics North 2008, 70990H (12 August 2008); https://doi.org/10.1117/12.807238

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