Optical cell monitoring system for underwater targets

SangJun Moon; Fahim Manzur; Tariq Manzur; Utkan Demirci

doi:10.1117/12.802761

16 October 2008 Optical cell monitoring system for underwater targets

SangJun Moon, Fahim Manzur, Tariq Manzur, Utkan Demirci

Proceedings Volume 7112, Unmanned/Unattended Sensors and Sensor Networks V; 71120Q (2008) https://doi.org/10.1117/12.802761
Event: SPIE Security + Defence, 2008, Cardiff, Wales, United Kingdom

Abstract

We demonstrate a cell based detection system that could be used for monitoring an underwater target volume and environment using a microfluidic chip and charge-coupled-device (CCD). This technique allows us to capture specific cells and enumerate these cells on a large area on a microchip. The microfluidic chip and a lens-less imaging platform were then merged to monitor cell populations and morphologies as a system that may find use in distributed sensor networks. The chip, featuring surface chemistry and automatic cell imaging, was fabricated from a cover glass slide, double sided adhesive film and a transparent Polymethlymetacrylate (PMMA) slab. The optically clear chip allows detecting cells with a CCD sensor. These chips were fabricated with a laser cutter without the use of photolithography. We utilized CD4⁺ cells that are captured on the floor of a microfluidic chip due to the ability to address specific target cells using antibody-antigen binding. Captured CD4⁺ cells were imaged with a fluorescence microscope to verify the chip specificity and efficiency. We achieved 70.2 ± 6.5% capturing efficiency and 88.8 ± 5.4% specificity for CD4⁺ T lymphocytes (n = 9 devices). Bright field images of the captured cells in the 24 mm × 4 mm × 50 μm microfluidic chip were obtained with the CCD sensor in one second. We achieved an inexpensive system that rapidly captures cells and images them using a lens-less CCD system. This microfluidic device can be modified for use in single cell detection utilizing a cheap light-emitting diode (LED) chip instead of a wide range CCD system.

Citation Download Citation

SangJun Moon, Fahim Manzur, Tariq Manzur, and Utkan Demirci "Optical cell monitoring system for underwater targets", Proc. SPIE 7112, Unmanned/Unattended Sensors and Sensor Networks V, 71120Q (16 October 2008); https://doi.org/10.1117/12.802761

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KEYWORDS

Microfluidics

Charge-coupled devices

Blood

Polymethylmethacrylate

CCD image sensors

Glasses

Imaging systems

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