Portable UV absorbance detection system for microchip electrophoresis

Li Tian; Wei J. Wang; Xiaowei Liu; Yu Song; Shaoyong Lu

doi:10.1117/12.807890

12 January 2009 Portable UV absorbance detection system for microchip electrophoresis

Li Tian, Wei J. Wang, Xiaowei Liu, Yu Song, Shaoyong Lu

Proceedings Volume 7133, Fifth International Symposium on Instrumentation Science and Technology; 71334G (2009) https://doi.org/10.1117/12.807890
Event: International Symposium on Instrumentation Science and Technology, 2008, Shenyang, China

Abstract

This paper presents a new structure of MCE chip on PMMA substrate prepared to improve the original chip structure with UV absorbance detection mode. The chip microchannels are fabricated by CNC milling and packaged with hot embossing process at the processing condition of 80°C, 5min, 0MPa with a manual powder pressing machine. All the micro-channels is 150&mgr;m * 150&mgr;m. Mobility &mgr;_eo of PMMA MCE chip is 2 9.15e-004cm Vs in the solution of 10mmol/L Na2CrO4 and 0.5mmol/L CTAB. Adopting indirect UV photometric detection mode, the inorganic anion Cl- capillary electrophoresis is carried out with an in-house UV detection system. The BGE buffer is 10 mmol/L Na2CrO4 and 0.5mmol/L CTAB, and the electrophoresis voltage is 616V. Test widow is 0.3*2 mm2. Test results indicate that both in-house UV detection system and MCE chip of PMMA substrate have the basic function and could be applied in the chemical analysis field.

Citation Download Citation

Li Tian, Wei J. Wang, Xiaowei Liu, Yu Song, and Shaoyong Lu "Portable UV absorbance detection system for microchip electrophoresis", Proc. SPIE 7133, Fifth International Symposium on Instrumentation Science and Technology, 71334G (12 January 2009); https://doi.org/10.1117/12.807890

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