Ex vivo and in vivo OCT image contrast

N. Krstajić; J. Jacobs; M. Bonesi; L. E. Smith; P. Deshpande; S. MacNeil; R. Smallwood; S. J. Matcher

doi:10.1117/12.819495

30 December 2008 Ex vivo and in vivo OCT image contrast

N. Krstajić, J. Jacobs, M. Bonesi, L. E. Smith, P. Deshpande, S. MacNeil, R. Smallwood, S. J. Matcher

Author Affiliations +

Proceedings Volume 7139, 1st Canterbury Workshop on Optical Coherence Tomography and Adaptive Optics; 71390W (2008) https://doi.org/10.1117/12.819495
Event: 1st Canterbury Workshop and School in Optical Coherence Tomography and Adaptive Optics, 2008, Canterbury, United Kingdom

Abstract

We present results of OCT and polarization-OCT applied to various ex vivo tissue samples and discuss related issues of image contrast, comparing in vivo and ex vivo preparations. Time-domain and frequency-domain OCT at 835nm and 1300nm have been applied to ex vivo skin and rabbit cornea. We can distinguish rabbit cornea epithelium for up to a month after excision. However, the skin loses all contrast upon excision and despite numerous experiments we cannot distinguish epidermis, which is clearly visible in vivo. Using a time-domain system, birefringence is clearly visible for decalcified tissue but can also be detected more weakly on fully mineralised tissue. Analysis suggests that demineralization increases the birefringence value.

Citation Download Citation

N. Krstajić, J. Jacobs, M. Bonesi, L. E. Smith, P. Deshpande, S. MacNeil, R. Smallwood, and S. J. Matcher "Ex vivo and in vivo OCT image contrast", Proc. SPIE 7139, 1st Canterbury Workshop on Optical Coherence Tomography and Adaptive Optics, 71390W (30 December 2008); https://doi.org/10.1117/12.819495

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