Raman microspectroscopy of optically trapped micro- and nanoobjects

Alexandr Jonáš; Jan Ježek; Mojmír Šerý; Pavel Zemánek

doi:10.1117/12.822382

20 November 2008 Raman microspectroscopy of optically trapped micro- and nanoobjects

Alexandr Jonáš, Jan Ježek, Mojmír Šerý, Pavel Zemánek

Proceedings Volume 7141, 16th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics; 714111 (2008) https://doi.org/10.1117/12.822382
Event: 16th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 2008, Polanica Zdroj, Poland

Abstract

We describe and characterize an experimental system for Raman microspectroscopy of micro- and nanoobjects optically trapped in aqueous suspensions with the use of a single-beam gradient optical trap (Raman tweezers). This system features two separate lasers providing light for the optical trapping and excitation of the Raman scattering spectra from the trapped specimen, respectively. Using independent laser beams for trapping and spectroscopy enables optimizing the parameters of both beams for their respective purposes. Moreover, it is possible to modulate the position of the trapped object relative to the Raman beam focus for maximizing the detected Raman signal and obtaining spatially resolved images of the trapped specimen. Using this experimental system, we have obtained Raman scattering spectra of individual optically confined micron and sub-micron sized polystyrene beads and baker's yeast cells. Sufficiently high signal-to-noise ratio of the spectra could be achieved using a few tens of milliwatts of the Raman beam power and detector integration times on the order of seconds.

Citation Download Citation

Alexandr Jonáš, Jan Ježek, Mojmír Šerý, and Pavel Zemánek "Raman microspectroscopy of optically trapped micro- and nanoobjects", Proc. SPIE 7141, 16th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 714111 (20 November 2008); https://doi.org/10.1117/12.822382

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