A universal label-free biosensing platform based on opto-fluidic ring resonators

Hongying Zhu; Ian M. White; Jonathan D. Suter; John Gohring; Xudong Fan

doi:10.1117/12.807792

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19 February 2009 A universal label-free biosensing platform based on opto-fluidic ring resonators

Hongying Zhu, Ian M. White, Jonathan D. Suter, John Gohring, Xudong Fan

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Proceedings Volume 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems; 71670I (2009) https://doi.org/10.1117/12.807792
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Rapid and accurate detection of biomolecules is important for medical diagnosis, pharmaceuticals, homeland security, food quality control, and environmental protection. A simple, low cost and highly sensitive label-free optical biosensor based on opto-fluidic ring resonator (OFRR) has been developed that naturally integrates microfluidics with ring resonators. The OFRR employs a piece of fused silica capillary with a diameter around 100 micrometers. The circular cross section of the capillary forms the ring resonator and light repeatedly travels along the resonator circumference in the form of whispering gallery modes (WGMs) through total internal reflection. When the capillary wall is as thin as a couple of micrometers (< 4 μm), an evanescent field of the WGMs exists at the OFRR inner surface and interacts with the sample when it flows through the OFRR. In order to detect the target molecules with high specificity, the OFRR inner surface is functionalized with receptors, such as antibodies, peptide-displayed bacteriophage or oligonucleotide DNA probes. The WGM spectral position shifts when biomolecules bind to the OFRR inner surface and change the local refractive index, which provides quantitative and kinetic information about the biomolecule interaction near the OFRR inner surface. The OFRR has been successfully demonstrated for detection of various types of biomoelcuels. Here, we will first introduce the basic operation principle of the OFRR as a sensor and then application examples of the OFRR in the detection of proteins, disease biomarkers, virus, DNA molecules, and cells with high sensitivities will be presented.

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Hongying Zhu, Ian M. White, Jonathan D. Suter, John Gohring, and Xudong Fan "A universal label-free biosensing platform based on opto-fluidic ring resonators", Proc. SPIE 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems, 71670I (19 February 2009); https://doi.org/10.1117/12.807792

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