Microflow cytometer

Frances S. Ligler; Jeffrey S. Erickson; Joel P. Golden; Jason S. Kim; Mansoor Nasir; Peter J. Howell Jr.; Abel L. Thangawng; Lisa Hilliard; George P. Anderson

doi:10.1117/12.807671

19 February 2009 Microflow cytometer

Frances S. Ligler, Jeffrey S. Erickson, Joel P. Golden, Jason S. Kim, Mansoor Nasir, Peter J. Howell Jr., Abel L. Thangawng, Lisa Hilliard, George P. Anderson

Author Affiliations +

Proceedings Volume 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems; 71670N (2009) https://doi.org/10.1117/12.807671
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

A multi-analyte diagnostic system based on a novel microflow cytometer is under development as a portable, fielddeployable sensor for environmental monitoring and for rapid point-of-care and on-site diagnosis of exposure to biothreat agents. The technology relies on a unique method for ensheathing a sample stream in continuous flow past an illuminated interrogation region. This sheathing approach efficiently focuses particles in the interrogation region of the fluidic channel and minimizes clogging by complex samples. Fluorescently coded microspheres provide the capability for highly multiplexed assays. In this report, separation of six microsphere sets was demonstrated with determination of immunoassays on three of the six sets; comparison to the commercial platform was made.

Citation Download Citation

Frances S. Ligler, Jeffrey S. Erickson, Joel P. Golden, Jason S. Kim, Mansoor Nasir, Peter J. Howell Jr., Abel L. Thangawng, Lisa Hilliard, and George P. Anderson "Microflow cytometer", Proc. SPIE 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems, 71670N (19 February 2009); https://doi.org/10.1117/12.807671

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