Narrow band imaging of tumors using gold nanoshells

Priyaveena Puvanakrishnan; Jaesook Park; Parameshwaran Diagaradjane; Jon A. Schwartz; Chris L. Coleman; Kelly L. Gill-Sharp; Kristina L. Sang; J. Donald Payne; Sunil Krishnan; James W. Tunnell

doi:10.1117/12.809613

23 February 2009 Narrow band imaging of tumors using gold nanoshells

Priyaveena Puvanakrishnan, Jaesook Park, Parameshwaran Diagaradjane, Jon A. Schwartz, Chris L. Coleman, Kelly L. Gill-Sharp, Kristina L. Sang, J. Donald Payne, Sunil Krishnan, James W. Tunnell

Author Affiliations +

Proceedings Volume 7169, Advanced Biomedical and Clinical Diagnostic Systems VII; 716912 (2009) https://doi.org/10.1117/12.809613
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Gold nanoshells (GNS) are a new class of nanoparticles that can be optically tuned to scatter or absorb light from the near-ultraviolet to near-infrared (NIR) region by varying the core (dielectric silica) /shell (gold) ratio. In addition to spectral tunability, GNS are inert and bioconjugatable making them potential labels for in vivo imaging and therapy of tumors. We report the use of GNS as exogenous contrast agents for enhanced visualization of tumors using narrow band imaging (NBI). NBI takes advantage of the strong NIR absorption of GNS to distinguish between blood and nanoshells in the tumor by imaging in narrow wavelength bands in the visible and NIR, respectively. Using tissue-simulating phantoms, we determined the optimum wavelengths to enhance contrast between blood and GNS. We then used the optimum wavelengths for ex-vivo imaging of tumors extracted from human colon cancer xenograft bearing mice injected with GNS. Systemically delivered GNS accumulated passively in tumor xenografts by the enhanced permeability and retention (EPR) effect. Ex-vivo NBI of tumor xenografts demonstrated tumor specific heterogeneous distribution of GNS with a clear distinction from the tumor vasculature. The results of this study demonstrate the feasibility of using GNS as contrast agents to visualize tumors using NBI.

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Priyaveena Puvanakrishnan, Jaesook Park, Parameshwaran Diagaradjane, Jon A. Schwartz, Chris L. Coleman, Kelly L. Gill-Sharp, Kristina L. Sang, J. Donald Payne, Sunil Krishnan, and James W. Tunnell "Narrow band imaging of tumors using gold nanoshells", Proc. SPIE 7169, Advanced Biomedical and Clinical Diagnostic Systems VII, 716912 (23 February 2009); https://doi.org/10.1117/12.809613

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