Affinity fluorescence-labeled peptides for the early detection of cancer in Barrett's esophagus

Meng Li; Shaoying Lu; Cyrus Piraka; Henry Appelman; Rich Kwon; Roy Soetikno; Tonya Kaltenbach; Thomas D. Wang

doi:10.1117/12.809140

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2 March 2009 Affinity fluorescence-labeled peptides for the early detection of cancer in Barrett's esophagus

Meng Li, Shaoying Lu, Cyrus Piraka, Henry Appelman, Rich Kwon, Roy Soetikno, Tonya Kaltenbach, Thomas D. Wang

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Proceedings Volume 7172, Endoscopic Microscopy IV; 71720J (2009) https://doi.org/10.1117/12.809140
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Fluorescence-labeled peptides that affinity bind to neoplastic mucsosa are promising for use as a specific contrast agent in the detection of pre-malignant tissue in the esophagus. This method is can be used to identify expression of biological markers associated with dysplasia on endoscopic imaging as a guide for biopsy and represents a novel method for the early detection and prevention of cancer. We demonstrate the use of phage display to select affinity peptides and identify the sequence "ASYNYDA" that binds with high target-to-background ratio to dysplastic esophageal mucosa compared to that of intestinal metaplasia. Validation of preferential binding is demonstrated for neoplasia in the setting of Barrett's esophagus. An optimal tradeoff between sensitivity and specificity of 82% and 85% was found at the relative threshold of 0.60 with a target-to-background ratio of 1.81 and an area under the ROC curve of 0.87. Peptides are a novel class of ligand for targeted detection of pre-malignant mucosa for purposes of screening and surveillance.

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Meng Li, Shaoying Lu, Cyrus Piraka, Henry Appelman, Rich Kwon, Roy Soetikno, Tonya Kaltenbach, and Thomas D. Wang "Affinity fluorescence-labeled peptides for the early detection of cancer in Barrett's esophagus", Proc. SPIE 7172, Endoscopic Microscopy IV, 71720J (2 March 2009); https://doi.org/10.1117/12.809140

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