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23 February 2009 Angular domain optical projection tomography in turbid media
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Most high-resolution optical tomography techniques employ coherence domain or time domain methodologies to capture non-scattered photons in turbid media. Angular Domain Optical Projection Tomography (ADOPT) uses an angular filter array (AFA) to observe photons that propagate through a specimen with small angular deviation. We constructed an ADOPT system consisting of an AFA micro-machined silicon micro-tunnel array with each micro-tunnel 60 μm wide, 60 μm high, 10 mm long, and separated by 5 μm thick walls. The range of acceptance angles was 0° to 0.5°. The system also included an 808 nm CW diode laser, beam shaping optics, a sample cuvette, a Keplerian lens system, and a CMOS camera. Testing was performed with a target consisting of two graphite rods (0.9 mm diameter) suspended in the cuvette by a rotation stage. The target was placed in a manner that the line of laser light was perpendicular to the long axis of the rods. A multitude of projections were collected at increments of 1.8° and compiled into a sinogram. A transverse image was reconstructed from the sinogram using filtered backprojection. The submillimeter targets embedded in the 2 cm thick scattering medium (reduced scattering coefficient ≤ 2.4 cm-1) were discernable in both the sinograms and the reconstructed images. The results suggest that ADOPT may be a useful technique for tomographic imaging of thick biological specimens (i.e. up to 8 mm across).
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Fartash Vasefi, Bozena Kaminska, Kevin Jordan, Glenn H. Chapman, and Jeffrey J. L. Carson "Angular domain optical projection tomography in turbid media", Proc. SPIE 7174, Optical Tomography and Spectroscopy of Tissue VIII, 71740D (23 February 2009);

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