Monitoring changes of proteins and lipids in laser welded aorta tissue using Raman spectroscopy and basis biochemical component analyses

C. H. Liu; W. B. Wang; A. Alimova; V. Sriramoju; V. Kartazayev; R. R. Alfano

doi:10.1117/12.809309

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24 February 2009 Monitoring changes of proteins and lipids in laser welded aorta tissue using Raman spectroscopy and basis biochemical component analyses

C. H. Liu, W. B. Wang, A. Alimova, V. Sriramoju, V. Kartazayev, R. R. Alfano

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Proceedings Volume 7175, Optical Interactions with Tissue and Cells XX; 717504 (2009) https://doi.org/10.1117/12.809309
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

The changes of Raman spectra from ex-vivo porcine aorta tissues were studied before and after laser tissue welding (LTW). Raman spectra were measured and compared for normal and welded tissues in both tunica adventitial and intimal sides. The vibrational modes at the peak of 1301 cm^-1 and the weak shoulder peak of 1264 cm^-1 of amide III for the normal tissue changed to a peak at 1322cm^-1 and a relative intense peak at 1264cm^-1, respectively, for the welded tissue. The Raman spectra were analyzed using a linear regression fitting method and compared with characteristic Raman spectra from proteins and lipids compounds. The relative biochemical molecular composition changes of proteins (Collagen types I, III, V and Elastin) and lipids for the laser welded tissue were modeled by basis biochemical component analyses (BBCA) and compared with the normal tissue.

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C. H. Liu, W. B. Wang, A. Alimova, V. Sriramoju, V. Kartazayev, and R. R. Alfano "Monitoring changes of proteins and lipids in laser welded aorta tissue using Raman spectroscopy and basis biochemical component analyses", Proc. SPIE 7175, Optical Interactions with Tissue and Cells XX, 717504 (24 February 2009); https://doi.org/10.1117/12.809309

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