The use of nanoparticles in medical treatment has prompted the question of their safety. In this study, the
pathophysiology and biodistribution of three different concentrations of intravenously-delivered dextran-coated Fe3O4
iron oxide nanoparticles (IONP) were evaluated in mice. Some groups of mice were exposed to an AC magnetic field
(AMF) at levels comparable with those proposed for cancer treatments. Iron biodistribution analysis for both AMF and
non-AMF treated mice was performed for all three concentrations used (.6 mg Fe/mouse, 1.8 mg Fe/mouse, and 5.6 mg
Fe/mouse). Blood urea nitrogen, alanine transaminase, alkaline phosphatase, total serum protein, and creatinine were
also assessed at 4 hours, 7 days, and 14 days post-injection. Histological analysis of lung, spleen, heart, liver, and kidney
tissue was conducted at 7 and 14 days post-injection. Prussian blue and H&E stains were used to histomorphometrically
assess iron content in the tissues studied. Preliminary results demonstrate small temporary elevation in liver enzymes and
hepatocyte vacuolization at all iron concentrations studied. Liver and spleen were the primary sites of IONP deposition.
None of the animals demonstrated systemic or local toxicity or illness, with or without AMF activation.
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