Analysis of NAD(P)H fluorescence components in cardiac myocytes from human biopsies: a new tool to improve diagnostics of rejection of transplanted patients

Y. Cheng; A. Mateasik; N. Poirier; J. Miró; N. Dahdah; D. Chorvat Jr.; A. Chorvatova

doi:10.1117/12.808001

25 February 2009 Analysis of NAD(P)H fluorescence components in cardiac myocytes from human biopsies: a new tool to improve diagnostics of rejection of transplanted patients

Y. Cheng, A. Mateasik, N. Poirier, J. Miró, N. Dahdah, D. Chorvat Jr., A. Chorvatova

Author Affiliations +

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 71830K (2009) https://doi.org/10.1117/12.808001
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Tissue autofluorescence is one of the most versatile non-invasive tools for mapping the metabolic state in living tissues. Increasing interest in the imaging and diagnosis of living cells and tissues, based on their intrinsic fluorescence rather than fluorescence labeling, is closely connected to the latest developments in high-performance spectroscopic and microscopic techniques. We investigate metabolic state of cardiac cells isolated from one additional human biopsy from transplanted pediatric patients presenting either no rejection (R0) or mild rejection (R1). Two different approaches for isolation of human cardiac myocytes are also compared. Spectrally-resolved fluorescence lifetime detection of NAD(P)H fluorescence (excitation by pulsed 375 nm picosecond laser) is tested as a promising new tool for quantitative analysis of intrinsic cellular autofluorescence signals in living cardiomyocytes. This work opens new horizons in the evaluation of cardiac transplant rejection using latest fluorescence imaging approaches.

Citation Download Citation

Y. Cheng, A. Mateasik, N. Poirier, J. Miró, N. Dahdah, D. Chorvat Jr., and A. Chorvatova "Analysis of NAD(P)H fluorescence components in cardiac myocytes from human biopsies: a new tool to improve diagnostics of rejection of transplanted patients", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71830K (25 February 2009); https://doi.org/10.1117/12.808001

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