Ultrafast multiphoton microscopy with high-order spectral phase distortion compensation

Yair Andegeko; Dmitry Pestov; Vadim V. Lozovoy; Marcos Dantus

doi:10.1117/12.808429

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13 February 2009 Ultrafast multiphoton microscopy with high-order spectral phase distortion compensation

Yair Andegeko, Dmitry Pestov, Vadim V. Lozovoy, Marcos Dantus

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Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 71830W (2009) https://doi.org/10.1117/12.808429
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

High-order dispersion of ultrashort laser pulses (with ~100 nm bandwidth) is shown to account for significant reduction of two-photon excitation fluorescence and second harmonic generation signal produced at the focal plane of a laser-scanning two-photon microscope. The second- and third-order corrections recover 20-40% of the signal intensity expected for a transform-limited laser pulse, while the rest depends on the proper compensation of higher-order terms. It can be accomplished through the use of a pulse shaper by measuring and correcting all nonlinear spectral phase distortions.

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Yair Andegeko, Dmitry Pestov, Vadim V. Lozovoy, and Marcos Dantus "Ultrafast multiphoton microscopy with high-order spectral phase distortion compensation", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71830W (13 February 2009); https://doi.org/10.1117/12.808429

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