Stimulated Raman scattering microscopy for biomedical imaging

Wei Min; Christian W. Freudiger; Sijia Lu; Chengwei He; Jing X. Kang; X. Sunney Xie

doi:10.1117/12.809802

13 February 2009 Stimulated Raman scattering microscopy for biomedical imaging

Wei Min, Christian W. Freudiger, Sijia Lu, Chengwei He, Jing X. Kang, X. Sunney Xie

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 718320 (2009) https://doi.org/10.1117/12.809802
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Label-free chemical contrast is highly desirable in biomedical imaging. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. Here we report a 3D multi-photon vibrational imaging technique based on stimulated Raman scattering (SRS). The sensitivity of SRS is significantly greater than that of spontaneous Raman scattering, and is further enhanced by high-frequency (MHz) phase-sensitive detection. SRS microscopy has a major advantage over previous coherent Raman techniques in that it offers background-free and easily interpretable chemical contrast. We show a variety of biomedical applications, such as differentiating distributions of omega-3 fatty acids and saturated lipids in living cells, imaging of brain and skin tissues based on intrinsic lipid contrast.

Citation Download Citation

Wei Min, Christian W. Freudiger, Sijia Lu, Chengwei He, Jing X. Kang, and X. Sunney Xie "Stimulated Raman scattering microscopy for biomedical imaging", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718320 (13 February 2009); https://doi.org/10.1117/12.809802

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