Transient state microscopy: a new tool for biomolecular imaging

Tor Sandén; Gustav Persson; Jerker Widengren

doi:10.1117/12.814908

13 February 2009 Transient state microscopy: a new tool for biomolecular imaging

Tor Sandén, Gustav Persson, Jerker Widengren

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 71832R (2009) https://doi.org/10.1117/12.814908
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Photoinduced transient dark states are exhibited by practically all common fluorophores. However, their information content has to date only been sparsely exploited due to methodological constraints. Here, a new concept is presented and verified that can monitor and image these states via their photodynamic fingerprints. It unites the environmental sensitivity of these states with the sensitivity of fluorescence-based detection. For demonstration, triplet state images of liposomes in different environments were generated, showing how local environmental differences can be resolved, not clearly distinguishable via other fluorescence parameters. The concept can provide several new, useful and independent fluorescence-based parameters in biomolecular imaging.

Citation Download Citation

Tor Sandén, Gustav Persson, and Jerker Widengren "Transient state microscopy: a new tool for biomolecular imaging", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71832R (13 February 2009); https://doi.org/10.1117/12.814908

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