SHIM and TPEM: getting more information from non linear excitation

Paolo Bianchini; Alberto Diaspro

doi:10.1117/12.809248

13 February 2009 SHIM and TPEM: getting more information from non linear excitation

Paolo Bianchini, Alberto Diaspro

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 718335 (2009) https://doi.org/10.1117/12.809248
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Several endogenous protein structures give rise to second harmonic generation (SHG) - second order nonabsorbative energy doubling of an excitation laser line. The orientation of collagen fibers within tissues such as tendons or ligaments is of primary importance. In this study, we propose a method to map the orientation of collagen fibers of a tendon. The method uses only few images acquired at specific polarizations of the input laser beam by rotating the sample on the stage. This procedure is implemented both in backward and forward scattering pathways. The improved details should clarify the backscattered signal nature.

Citation Download Citation

Paolo Bianchini and Alberto Diaspro "SHIM and TPEM: getting more information from non linear excitation", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718335 (13 February 2009); https://doi.org/10.1117/12.809248

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