FPGA-based electronics for confocal line scanners with linear detector arrays

Sanjee Abeytunge; Ricardo Toledo-Crow; Milind Rajadhyaksha

doi:10.1117/12.809423

4 March 2009 FPGA-based electronics for confocal line scanners with linear detector arrays

Sanjee Abeytunge, Ricardo Toledo-Crow, Milind Rajadhyaksha

Proceedings Volume 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI; 71840A (2009) https://doi.org/10.1117/12.809423
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

One-dimensional linear detector arrays have been used in the development of microscopes. Our confocal line scanning microscope electronics incorporate two printed circuit boards: control board and detector board. This architecture separates control electronics from detection electronics allowing us to minimize the footprint at microscope detector head. The Field Programmable Gate array (FPGA) on the control board generates timing and synchronization signals to three systems: detector board, frame grabber and galvanometric mirror scanner. The detector is kept away from its control electronics, and the clock and control signals are sent over a differential twisted-pair cable. These differential signals are translated to single ended signals and forwarded to the detector at the microscope detector head. The synchronization signals for the frame grabber are sent over a shielded cable. The control board also generates a saw tooth analog ramp to drive the galvanometric mirror scanner. The analog video output of the detector is fed into an operational amplifier where the white and the black levels are adjusted. Finally the analog video is send to the frame grabber via a shielded cable. FPGA-based electronics offer an inexpensive convenient means to control and synchronize simple line-scanning confocal microscopes.

Citation Download Citation

Sanjee Abeytunge, Ricardo Toledo-Crow, and Milind Rajadhyaksha "FPGA-based electronics for confocal line scanners with linear detector arrays", Proc. SPIE 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI, 71840A (4 March 2009); https://doi.org/10.1117/12.809423

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KEYWORDS

Sensors

Clocks

Signal detection

Electronics

Field programmable gate arrays

Microscopes

Analog electronics

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