Fluorescence microscopy with a coded aperture snapshot spectral imager

Christy A. Fernandez; Ashwin Wagadarikar; David J. Brady; Scott C. McCain; Tim Oliver

doi:10.1117/12.807863

3 March 2009 Fluorescence microscopy with a coded aperture snapshot spectral imager

Christy A. Fernandez, Ashwin Wagadarikar, David J. Brady, Scott C. McCain, Tim Oliver

Proceedings Volume 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI; 71840Z (2009) https://doi.org/10.1117/12.807863
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

This paper describes the application of a coded aperture snapshot spectral imager (CASSI) to fluorescence microscopy. CASSI records an interleaved spatially varying, spectrally filtered map of an object on a 2D focal plane. Convex optimization techniques combining least squares QR factorization with a total variance constraint are used to reconstruct a 3D data cube from a spectrally encoded 2D scene. CASSI records a 3D dataset at video rate - making it suitable for dynamic cellular imaging. We report on the reconstruction of fluorescent microspheres used in fluorescence microscopy applications and compare the results with images from a multi-spectral confocal system.

Citation Download Citation

Christy A. Fernandez, Ashwin Wagadarikar, David J. Brady, Scott C. McCain, and Tim Oliver "Fluorescence microscopy with a coded aperture snapshot spectral imager", Proc. SPIE 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI, 71840Z (3 March 2009); https://doi.org/10.1117/12.807863

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