Temperature impact on thermodynamical parameters of DNA:DNA hybridization kinetics as quantified by Surface Plasmon Resonance Imaging (SPRI) system

Jérome Hottin; Julien Moreau; Michael Canva

doi:10.1117/12.808966

20 March 2009 Temperature impact on thermodynamical parameters of DNA:DNA hybridization kinetics as quantified by Surface Plasmon Resonance Imaging (SPRI) system

Jérome Hottin, Julien Moreau, Michael Canva

Author Affiliations +

Proceedings Volume 7192, Plasmonics in Biology and Medicine VI; 71920C (2009) https://doi.org/10.1117/12.808966
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Surface plasmon resonance imaging optical biochips have shown promising applications in the field of genetic diagnosis due to their ability to record hundred of different biomolecular interactions without any label and in real time. Single nucleotide polymorphisms can be detected using this technique. To improve the accuracy and the reliability of biochips a quantitative study on the impact of temperature on DNA hybridization and secondary structures is shown for various oligonucleotides sequences. In this proceeding we focus on the impact of temperature T on the binding rate kinetic τ, using the model case of DNA:DNA interactions. We show that SPRI can quantify such characteristics as τ(T) and that they follow the Langmuir model variation law.

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Jérome Hottin, Julien Moreau, and Michael Canva "Temperature impact on thermodynamical parameters of DNA:DNA hybridization kinetics as quantified by Surface Plasmon Resonance Imaging (SPRI) system", Proc. SPIE 7192, Plasmonics in Biology and Medicine VI, 71920C (20 March 2009); https://doi.org/10.1117/12.808966

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