Observation of living cells with gold nanoparticles by using surface-enhanced Raman scattering

Katsumasa Fujita; Jun Ando; Sawako Ishitobi; Keisaku Hamada; Nicholas I. Smith; Yasushi Inouye; Satoshi Kawata

doi:10.1117/12.809115

18 February 2009 Observation of living cells with gold nanoparticles by using surface-enhanced Raman scattering

Katsumasa Fujita, Jun Ando, Sawako Ishitobi, Keisaku Hamada, Nicholas I. Smith, Yasushi Inouye, Satoshi Kawata

Proceedings Volume 7192, Plasmonics in Biology and Medicine VI; 71920Q (2009) https://doi.org/10.1117/12.809115
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We observed spatial and temporal behaviors of surface enhanced Raman scattering (SERS) signals with gold nanoparticles in living cells. Gold nanoparticles with the diameter of 50 nm were introduced into macrophage cells through endocytosis. We performed observation of SERS signals from a macrophage with 785 nm excitation. Strong SERS signal from the particles in the cell was observed, and spectrum from each particle shows difference in Raman peaks and intensity. We also observed time-lapse SERS and dark-field image with a frame rate of 3 min. We confirmed that strong SERS signal from the particle in the cell and the spectral changes with positions of the particles in the cell.

Citation Download Citation

Katsumasa Fujita, Jun Ando, Sawako Ishitobi, Keisaku Hamada, Nicholas I. Smith, Yasushi Inouye, and Satoshi Kawata "Observation of living cells with gold nanoparticles by using surface-enhanced Raman scattering", Proc. SPIE 7192, Plasmonics in Biology and Medicine VI, 71920Q (18 February 2009); https://doi.org/10.1117/12.809115

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