High-throughput on-chip in vivo neural regeneration studies using femtosecond laser nano-surgery and microfluidics

Christopher B. Rohde; Fei Zeng; Cody Gilleland; Chrysanthi Samara; Mehmet F. Yanik

doi:10.1117/12.822328

24 February 2009 High-throughput on-chip in vivo neural regeneration studies using femtosecond laser nano-surgery and microfluidics

Christopher B. Rohde, Fei Zeng, Cody Gilleland, Chrysanthi Samara, Mehmet F. Yanik

Author Affiliations +

Proceedings Volume 7203, Commercial and Biomedical Applications of Ultrafast Lasers IX; 72030E (2009) https://doi.org/10.1117/12.822328
Event: SPIE LASE: Lasers and Applications in Science and Engineering, 2009, San Jose, California, United States

Abstract

In recent years, the advantages of using small invertebrate animals as model systems for human disease have become increasingly apparent and have resulted in three Nobel Prizes in medicine or chemistry during the last six years for studies conducted on the nematode Caenorhabditis elegans (C. elegans). The availability of a wide array of species-specific genetic techniques, along with the transparency of the worm and its ability to grow in minute volumes make C. elegans an extremely powerful model organism. We present a suite of technologies for complex high-throughput whole-animal genetic and drug screens. We demonstrate a high-speed microfluidic sorter that can isolate and immobilize C. elegans in a well-defined geometry, an integrated chip containing individually addressable screening chambers for incubation and exposure of individual animals to biochemical compounds, and a device for delivery of compound libraries in standard multiwell plates to microfluidic devices. The immobilization stability obtained by these devices is comparable to that of chemical anesthesia and the immobilization process does not affect lifespan, progeny production, or other aspects of animal health. The high-stability enables the use of a variety of key optical techniques. We use this to demonstrate femtosecond-laser nanosurgery and three-dimensional multiphoton microscopy. Used alone or in various combinations these devices facilitate a variety of high-throughput assays using whole animals, including mutagenesis and RNAi and drug screens at subcellular resolution, as well as high-throughput high-precision manipulations such as femtosecond-laser nanosurgery for large-scale in vivo neural degeneration and regeneration studies.

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Christopher B. Rohde, Fei Zeng, Cody Gilleland, Chrysanthi Samara, and Mehmet F. Yanik "High-throughput on-chip in vivo neural regeneration studies using femtosecond laser nano-surgery and microfluidics", Proc. SPIE 7203, Commercial and Biomedical Applications of Ultrafast Lasers IX, 72030E (24 February 2009); https://doi.org/10.1117/12.822328

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KEYWORDS

Microfluidics

Genetics

Animal model studies

Multiphoton microscopy

Neurons

Femtosecond phenomena

In vivo imaging

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