Fast and precise detection of ricin with microcapillary sensor system

Jun-Tae Lee; Dosi Dosev; Mikaela Nichkova; Zhiya Ma; Shirley Gee; Bruce D. Hammock; Ian M. Kennedy

doi:10.1117/12.809421

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23 February 2009 Fast and precise detection of ricin with microcapillary sensor system

Jun-Tae Lee, Dosi Dosev, Mikaela Nichkova, Zhiya Ma, Shirley Gee, Bruce D. Hammock, Ian M. Kennedy

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Proceedings Volume 7207, Microfluidics, BioMEMS, and Medical Microsystems VII; 72070G (2009) https://doi.org/10.1117/12.809421
Event: SPIE MOEMS-MEMS: Micro- and Nanofabrication, 2009, San Jose, California, United States

Abstract

Ricin is an easily available toxin which can be used as a bio-terror agent. Fast and inexpensive methods for its detection in different samples are needed. Recently we have developed a novel fluorescent sandwich immunoassay for ricin using magnetic-luminescent nanoparticles (MLNPs) as carriers in a microcapillary system for incubation and detection. Antiricin antibody coated MLNPs that were dispersed in buffer solution were introduced in the capillary tube and immobilized inside using an external electromagnet. Then the sample containing ricin was injected while the MLNPs were mixed by an alternating magnetic field. After the incubation, washing solution and secondary antibody conjugated with Alexa-fluorescent were injected into the capillary while the MLNPs were constantly mixed. After the final wash, the particles were immobilized for detection. The total analysis time was reduced to less than forty minutes which is about 8-10 fold improvement in comparison with the plate-based protocols. This system is promising for the development of a portable biosensor and can be used for the detection of other analytes of interest.

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Jun-Tae Lee, Dosi Dosev, Mikaela Nichkova, Zhiya Ma, Shirley Gee, Bruce D. Hammock, and Ian M. Kennedy "Fast and precise detection of ricin with microcapillary sensor system", Proc. SPIE 7207, Microfluidics, BioMEMS, and Medical Microsystems VII, 72070G (23 February 2009); https://doi.org/10.1117/12.809421

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